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Hierarchical All-against-All association testing (HAllA) is computational method to find multi-resolution associations in high-dimensional, heterogeneous datasets.
HAllA is an end-to-end statistical method for Hierarchical All-against-All discovery of significant relationships among data features with high power. HAllA is robust to data type, operating both on continuous and categorical values, and works well both on homogeneous datasets (where all measurements are of the same type, e.g. gene expression microarrays) and on heterogeneous data (containing measurements with different units or types, e.g. patient clinical metadata). Finally, it is also aware of multiple input, multiple output problems, in which data might contain of two (or more) distinct subsets sharing an index (e.g. clinical metadata, genotypes, microarrays, and microbiomes all drawn from the same subjects). In all of these cases, HAllA will identify which pairs of features (genes, microbes, loci, etc.) share statistically significant information, without getting tripped up by high-dimensionality.
For more information on the technical aspects:
User Manual || User Tutorial || Forum
Citation:
Gholamali Rahnavard, Eric A. Franzosa, Lauren J. McIver, Emma Schwager, Jason Lloyd-Price, George Weingart, Yo Sup Moon, Xochitl C. Morgan, Levi Waldron, Curtis Huttenhower, 'High-sensitivity pattern discovery in large multi'omic datasets'.
In short, HAllA is like testing for correlation among all pairs of variables in a high-dimensional dataset, but without tripping over multiple hypothesis testing, the problem of figuring out what 'relation or association' means for different units or scales, or differentiating between predictor/input or response/output variables.
Its advantages include:
- Generality: HAllA can handle datasets of mixed data types: categorical, binary, or continuous.
- Efficiency: Rather than checking all possible possible associations, HAllA prioritizes computation such that only statistically promising candidate variables are tested in detail.
- Reliability: HAllA utilizes hierarchical false discovery correction to limit false discoveries and loss of statistical power attributed to multiple hypothesis testing.
- Extensibility: HAllA is extensible to use different methods so measurement in its steps.
- Similarity measurement it has the following metrics implemented: normalized mutual information (NMI), Spearman correlation, Pearson correlation, xicor (a.k.a. Chatterjee correlation), and distance correlation (DCOR).
- Dimension reduction - the features in each input dataset are connected by a hierarchical tree according to their similarity. This acts as a simple way of connecting co-varying features into a coherent 'block'.
- False discovery rate correction (FDR) methods are included: Benjamini–Hochberg–Yekutieli (BHY) as defualt, Benjamini–Hochberg (BH), Bonferroni.
Operating System
Software
- Option 1 (Recommended):
pip install halla - Option2:
- Download and unpack the latest release of HAllA.
- Unpack the HAllA software:
tar -ztvf halla.tar.gz - Move to the HAllA directory :
$ cd halla - Install HAllA:
$ python setup.py install
Sph_ Mac Os X
Note: If you do not have write permissions to ‘/usr/lib/', then add the option '–user' to the install command. This will install the python package into subdirectories of ‘~/.local'.
Type the command:
- General command:
$ halla -X $DATASET1 -Y $DATASET2 --output $OUTPUT_DIR - Example:
$ halla -X X_parabola_F64_S50.txt -Y Y_parabola_F64_S50.txt -o HAllA_OUTPUT
HAllA by default takes two tab-delimited text files as an input, where in each file, each row describes feature (data/metadata) and each column represents an instance. In other words, input X is a D x N matrix where D is the number of dimensions in each instance of the data and N is the number of instances (samples). The 'edges' of the matrix should contain labels of the data, if desired.
Note: the input files have the same samples(columns) but features(rows) could be different.
HAllA by default writes the results to 'associations.txt', a tab-delimited text file as output for significant association: Trains & things (itch) mac os.
$OUTPUT_DIR = the output directory
Sph_ Mac Os 11
Operating System
Software
- Option 1 (Recommended):
pip install halla - Option2:
- Download and unpack the latest release of HAllA.
- Unpack the HAllA software:
tar -ztvf halla.tar.gz - Move to the HAllA directory :
$ cd halla - Install HAllA:
$ python setup.py install
Sph_ Mac Os X
Note: If you do not have write permissions to ‘/usr/lib/', then add the option '–user' to the install command. This will install the python package into subdirectories of ‘~/.local'.
Type the command:
- General command:
$ halla -X $DATASET1 -Y $DATASET2 --output $OUTPUT_DIR - Example:
$ halla -X X_parabola_F64_S50.txt -Y Y_parabola_F64_S50.txt -o HAllA_OUTPUT
HAllA by default takes two tab-delimited text files as an input, where in each file, each row describes feature (data/metadata) and each column represents an instance. In other words, input X is a D x N matrix where D is the number of dimensions in each instance of the data and N is the number of instances (samples). The 'edges' of the matrix should contain labels of the data, if desired.
Note: the input files have the same samples(columns) but features(rows) could be different.
HAllA by default writes the results to 'associations.txt', a tab-delimited text file as output for significant association: Trains & things (itch) mac os.
$OUTPUT_DIR = the output directory
Sph_ Mac Os 11
$OUTPUT_DIR/associations.txt
Sph_ Mac Os 11
- Each row of the assocation.txt tab-delimited file has the following information for each association:
- association_rank: association are sorted for significancy by low pvalues and high similarity score.
- cluster1: has one or more homogenous features from the first dataset that participate in the association.
- cluster1_similarity_score: this value is correspond to `1 – condensed distance` of cluster in the hierarchy of the first dataset.
- cluster2: has one or more homogenous features from the second dataset that participate in the association.
- cluster2_similarity_score: this value is correspond to `1 – condensed distance` of cluster in the hierarchy of the second dataset.
- pvalue : p-value from Benjamini-Hochberg-Yekutieli approach used to assess the statistical significance of the mutual information distance.
- qvalue: q value calculates after BHY correction for each test.
- similarity_score_between_clusters: is the similarity score of the representatives (medoids) of two clusters in the association.
- HAllA provides several plots as complementary outputs including: hallagram for overall plotting results, diagnostics-plot for each association plotting, and heatmaps of original input datasets.
